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There is a need for RNA amplification and library preparation techniques that can be applied to pico-quantities of total RNA to properly understand the role of small RNA in diseases and cell development. SUBREE SUBRAMANIAN Professor, University of Minnesota and Chief Scientific Officer at EV Therapeutics 1y Happy to share our recent publication describing a novel mechanism of immune evasion in. Amplification and library preparation methods are restricted due to the limited template RNA, and small RNA profiles have been unable to be produced. However, these cells are limited in number, and are therefore difficult to dissect and analyze. Diseases in tissue often have trigger cells that directly contribute to the propagation of secondary cells, and these trigger cells have RNA which is of particular use in research. Currently, understanding of RNA is limited due to the scarcity of cells available to use in experimentation. The expression of certain RNA segments has been shown to determine the health of cells and whether they are likely to express diseased qualities. Price New from Used from Hardcover, Decem'Please retry' 81.00 76.79 65.10 Hardcover81.00 1 Used from65.10 3 New from76. Please contact us for specific details.Ĭritical disease and cellular functions are closely linked to small RNAs such as miRNAs, endo-siRNAs and piRNAs. by Subree Subramanian(Editor), Xianda Zhao(Editor) See all formats and editions Sorry, there was a problem loading this page.

#SUBREE SUBRAMANIAN LICENSE#

This technology is available via a standard negotiated license agreement. This library preparation of small RNA is the first to accomplish small RNA profiling and is more costĮffective than current methods. A genome-wide approach to comparative oncology: High-resolution oligonucleotide aCGH of canine and human osteosarcoma pinpoints shared microaberrations. The efficiency of preparation and amplification allows for small segments of RNA from scarce cells to be appropriately analyzed along with longer RNA fragments, which is an improvement over other methods which require small and long RNAs to be amplified and profiled in separate experiments. Angstadt AY, Thayanithy V, Subramanian S, Modiano JF, and Breen M. The amplification requires only 500pg to 5ng for a template and can capture RNA of 15 nucleotides and longer. This system is the first of its kind to properly amplify small quantities of RNA as a template while still producing a highly efficient library.

University of Minnesota researchers have developed an amplification method and library preparation process of pico-quantities of RNA. Title: Host-microRNA-microbiota interactions in colorectal cancer. IP Status: Issued US Patent Application #: 15/100,784 Small RNA Profiling of Surgery, University of Minnesota, USA).

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